The effects of low pH and CO2 on bovine corneal lactate production

Hypoxic corneal swelling in human subjects is reduced by CO2 induced corneal acidosis. We investigated whether this effect could be explained by a reduction in lactate production under acidic conditions. Lactate production from isolated bovine corneas was assayed under normoxic and hypoxic condition...

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Bibliographic Details
Published inThe CLAO journal Vol. 23; no. 4; p. 282
Main Authors Sarroca, A, Lin, S, Bonanno, J A
Format Journal Article
LanguageEnglish
Published United States 01.10.1997
Subjects
Acidosis - metabolism
Acidosis - pathology
Animals
Carbon Dioxide - metabolism
Cattle
Cornea - cytology
Cornea - metabolism
Hydrogen-Ion Concentration
Hypoxia - metabolism
Hypoxia - pathology
Lactic Acid - biosynthesis
Spectrophotometry
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Summary:Hypoxic corneal swelling in human subjects is reduced by CO2 induced corneal acidosis. We investigated whether this effect could be explained by a reduction in lactate production under acidic conditions. Lactate production from isolated bovine corneas was assayed under normoxic and hypoxic conditions at normal pH (7.5) and low pH (7.05). Low pH was achieved using a CO2/HCO3- Ringer or a HEPES buffered Ringer. Normoxic corneas produced significantly less (approximately 25%) lactate under all acidic conditions. In contrast, hypoxic corneas produced the same or slightly less lactate under acidic conditions (weighted average of all conditions = 6% less lactate). From these results, we concluded that pH can have a substantial effect on lactate production, but its influence on glycolytic activity during hypoxia is suppressed and may be negligible.
ISSN:0733-8902