F2-isoprostane and 4-hydroxynonenal excretion in human bile of patients with biliary tract and pancreatic disorders

• Published in: The American journal of gastroenterology Vol. 92; no. 11; p. 2069
• Main Authors: Leo, M A, Aleynik, S I, Siegel, J H, Kasmin, F E, Aleynik, M K, Lieber, C S
• Format: Journal Article
• Language: English
• Published: United States 01.11.1997
• Subjects: Aged; Aldehydes - analysis; Aldehydes - metabolism; Analysis of Variance; beta Carotene - analysis; Bile - chemistry; Bile - metabolism; Bile Duct Diseases - diagnostic imaging; Bile Duct Diseases - metabolism; Cholangiopancreatography, Endoscopic Retrograde; Cholelithiasis - diagnostic imaging; Cholelithiasis - metabolism; Chromatography, High Pressure Liquid; Dinoprost - analogs & derivatives; Dinoprost - analysis; Dinoprost - metabolism; Gas Chromatography-Mass Spectrometry; Humans; Middle Aged; Pancreatic Diseases - diagnostic imaging; Pancreatic Diseases - metabolism; Vitamin E - analysis
• ISSN: 0002-9270

To assess parameters of lipid peroxidation in bile of patients with hepatobiliary and pancreatic diseases. F2-isoprostanes (F2-IPs) and 4-hydroxynonenal (4-HNE) were measured in bile collected during 31 ERCP procedures using gas chromatography/mass spectrometry. In 11 subjects with normal ERCP (controls), bile contained significant amounts of F2-IPs (188 +/- 27 pg/ml) and 4-HNE (37.5 +/- 8.0 ng/ml). In 10 individuals with bile duct stones, there was a 3-fold increase of F2-IPs (523 +/- 129 pg/ml; p < 0.05) and a 2.5-fold increase of 4-HNE (89.6 +/- 18.0 ng/ml; p < 0.05). In 10 patients with various pancreatic diseases, bile F2-IPs were also enhanced (545 +/- 112 pg/ml; p < 0.01). There was no significant change in alpha-tocopherol, whereas beta-carotene was decreased in biliary tract and pancreatic diseases (p < 0.05). Results of serum liver tests were normal with the exception of bilirubin, which was increased together with alkaline phosphatase. Concentrations of total lipids, phospholipids, and cholesterol did not differ significantly between the three groups. These data provide the first evidence in humans supporting the role of oxidative stress in the pathogenesis of biliary and pancreatic disease.