Primers for Salmonella serovar detection by polymerase chain reaction

• Published in: Southeast Asian journal of tropical medicine and public health Vol. 29; no. 1; pp. 85 - 90
• Main Authors: KANTAMA, L, JAYANETRA, P, PILANTANAPAK, A, CHARAENSUB, A
• Format: Journal Article
• Language: English
• Published: Bangkok Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network 01.03.1998
• Subjects: Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Base Sequence; Biological and medical sciences; DNA Primers - genetics; Human bacterial diseases; Humans; Infectious diseases; Medical sciences; Molecular Sequence Data; Polymerase Chain Reaction - methods; Salmonella - genetics; Salmonella Infections - diagnosis; Sensitivity and Specificity; Tropical medicine; Thailand; Asia; Infection; Human; Interest; Salmonella; Food industry; Bacteriosis; Bacteria; Salmonellosis; Diagnosis; Medical screening; Enterobacteriaceae
• ISSN: 0125-1562

Salmonella serovar detection was studied by polymerase chain reaction (PCR). The primers were designed from Salmonella specific clone, A18:2 which was previously constructed and studied for genus specificity through colony hybridization. The primers were subsequently tested for specificity and sensitivity and showed that they amplified DNA fragment of all Salmonellae tested but did not amplify all isolates of non-Salmonellae tested. The amplified fragment was confirmed and increased sensitivity by nested PCR. Salmonella isolates amplified by the primers in the first round PCR were all positive in the second round. The sensitivity in the first and second round were 7 pg and 80 fg, respectively. The result indicated that the primers can be used as molecular tool for future field survey of Salmonella both in food and in clinical specimens.