Pyridine nucleotide synthesis in human blood--effect of leucine, alpha-ketoisocaproic acid and ketone bodies

The effects of L-leucine and its metabolites on pyridine nucleotide synthesis were investigated in human blood. After 30 min-incubation 40% of the added (carboxyl-14C)-nicotinic acid was incorporated into pyridine nucleotides, most of which was nicotinic acid mononucleotide. Leucine and alpha-ketois...

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Bibliographic Details
Published inInternational journal for vitamin and nutrition research Vol. 53; no. 4; p. 432
Main Authors Yamada, O, Kiyohara, Y, Sano, K, Umezawa, C
Format Journal Article
LanguageEnglish
Published Switzerland 1983
Subjects
Adenine Nucleotides - biosynthesis
Adenine Nucleotides - blood
Humans
Keto Acids - pharmacology
Ketone Bodies - pharmacology
Leucine - pharmacology
NAD - biosynthesis
NAD - blood
NADP - blood
Niacin - metabolism
Nicotinamide Mononucleotide - blood
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ISSN0300-9831

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Summary:The effects of L-leucine and its metabolites on pyridine nucleotide synthesis were investigated in human blood. After 30 min-incubation 40% of the added (carboxyl-14C)-nicotinic acid was incorporated into pyridine nucleotides, most of which was nicotinic acid mononucleotide. Leucine and alpha-ketoisocaproic acid reduced pyridine nucleotide synthesis by 40% and 15%, respectively at 1 mM level. Ketone bodies were without effect. The results were different from those observed in rat hepatocytes [O. Yamada et al.: Internat. J. Vitam. Nutr. Res. 53, 184 (1983)], in which ketone bodies and the compounds metabolized to ketone bodies were inhibitory to the NAD biosynthesis.
ISSN:0300-9831