ANTIBIOTIC RESISTANCE OF ESCHERICHIA COLI ISOLATED FROM UKRAINIAN BETULA VERRUCOSA EHRH. POLLEN AFTER MICROBIOLOGICAL ANALYSIS

Seven samples of silver birch pollen from different habitat of Ukraine were investigated in order to estimate their contamination with the Enterobacteriaceae family, anaerobic bacteria and fungi. Also resistance of 108 strains of Escherichia coli isolated from seven samples of Ukrainian Betula verru...

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Published inJournal of microbiology, biotechnology and food sciences Vol. 3; no. 1; pp. 94 - 96
Main Authors Shevtsova, Tetiana, Hleba, Lukás, Kacániová, Miroslava, Brindza, Ján, Garkava, Kateryna
Format Journal Article
LanguageEnglish
Published Nitra Faculty of Biotechnology and Food Sciences 01.08.2013
Subjects
Antibiotics
Bacteria
Betula verrucosa
Drug resistance
E coli
Enterobacteriaceae
Escherichia coli
Fungi
Microorganisms
Kyiv Ukraine
Ukraine
Slovakia
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Summary:Seven samples of silver birch pollen from different habitat of Ukraine were investigated in order to estimate their contamination with the Enterobacteriaceae family, anaerobic bacteria and fungi. Also resistance of 108 strains of Escherichia coli isolated from seven samples of Ukrainian Betula verrucosa Ehrh. pollen against 5 antibiotics: ampicillin, chloramphenicol, meropenem, ceftriaxone and ofloxacin were determined. Disc diffusion method was used for antibiotic suceptibility testing according to EUCAST 2012. It is established the concentrations of enterobacteria ranged from 0.00 to 4.16 log cfu/g, of anaerobic bacteria - 2.48 to 4.90 log cfu/g and concentration of fungi ranged from 2.48 to 4.14 log cfu/g. Degree of pollen contamination is different depending on the habitats. The resistance of E. coli isolates was determined against ampicillin, chloramphenicol, meropenem and ofloxacin. But intermediate resistance in the 33.3% of E. coli isolates and susceptibility in the 8.3% to ceftriaxone was found out. Antibiotic resistance was evaluated for all samples of pollen in whole.
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ISSN:1338-5178