The maize transposable element Mu1 does not transpose in tobacco

The maize transposable element Mu1 was introduced into Nicotiana tabacum by a Ti plasmid-derived binary vector of Agrobacterium tumefaciens. Forty-eight independent kanamycin resistant shoots were obtained by the leaf disk transformation-regeneration method. Southern blot analysis of transformed pla...

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Published inMaydica Vol. 36; no. 3; pp. 197 - 203
Main Authors DEL GIUDICE, L, MANNA, F, MASSARDO, D.R, PIROVANO, L, DE PRISCO, R, SORRENTINO, C, TONINI, A, WOLF, K
Format Journal Article
LanguageEnglish
Published Bergamo Maydica 1991
Subjects
Agronomy. Soil science and plant productions
Biological and medical sciences
Classical and quantitative genetics. Population genetics. Molecular genetics
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics and breeding of economic plants
Molecular genetics
Nicotiana tabacum
Zea mays
Monocotyledones
Cauliflower mosaic virus
Zea mays
Stimulant plant
Transposable element
Gene expression
Nicotiana tabacum
Cereal crop
Virus
Gene
Caulimovirus
Gramineae
Dicotyledones
Angiospermae
Genetics
Spermatophyta
Solanaceae
Mutation
Molecular biology
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Summary:The maize transposable element Mu1 was introduced into Nicotiana tabacum by a Ti plasmid-derived binary vector of Agrobacterium tumefaciens. Forty-eight independent kanamycin resistant shoots were obtained by the leaf disk transformation-regeneration method. Southern blot analysis of transformed plants revealed that the Mu1-DNA remained entirely within the T-DNA. This was the case during four sexual generations and also during dedifferentiation/redifferentiation regimes of callus cultures and regenerated plants.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0025-6153
2279-8013