Topoisomerase IIα and topoisomerase IIβ genes : characterization and mapping to human chromosomes 17 and 3, respectively

• Published in: Cancer research (Chicago, Ill.) Vol. 52; no. 1; pp. 231 - 234
• Main Authors: TAN, K. B, DORMAN, T. E, FALLS, K. M, CHUNG, T. D. Y, MIRABELLI, C. K, CROOKE, S. T, JEI-I MAO
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 1992
• Subjects: Analytical, structural and metabolic biochemistry; Biological and medical sciences; Blotting, Southern; chromosome 17; chromosome 3; Chromosome Mapping; Chromosomes, Human, Pair 17 - enzymology; Chromosomes, Human, Pair 3 - enzymology; DNA topoisomerase (ATP-hydrolysing); DNA topoisomerase (ATP-hydrolysing) alpha; DNA topoisomerase (ATP-hydrolysing) beta; DNA Topoisomerases, Type II - genetics; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Humans; Isoenzymes - genetics; Isomerases; Genetic mapping; Human; DNA topoisomerase (ATP-hydrolysing); Complementary DNA; Gene; Enzyme; Isozyme; Molecular cloning; Southern blotting; Polymorphism
• ISSN: 0008-5472; 1538-7445

Human cells contain two topoisomerase II isozymes named topo II alpha and topo II beta. The complementary DNAs for both enzymes have been cloned. The topo II alpha and topo II beta complementary DNAs hybridized to unique sequences of human, rodent, and chicken DNAs in Southern blots. The human topo II alpha gene has previously been mapped to chromosome 17. We confirmed the chromosomal location of topo II alpha and mapped the topo II beta gene to chromosome 3. In addition, topo II beta exhibits genetic polymorphism as has been reported for topoisomerases I and II alpha.