Soil bacterial community diversity and life strategy during slope restoration on an uninhabited island: changes under the aggregate spray-seeding technique

• Published in: Journal of applied microbiology Vol. 135; no. 6
• Main Authors: Wang, Zhikang, Zhang, Shilei, Geng, Zengchao, Li, Chunlin, Sun, Linting, Zhang, Liangzhen, Cao, Zhiquan
• Format: Journal Article
• Language: English
• Published: England 03.06.2024
• Subjects: Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biodiversity; DNA, Bacterial - genetics; Islands; Seasons; Soil - chemistry; Soil Microbiology; diversity; soil bacterial community; life strategy; restoration
• ISSN: 1365-2672; 1365-2672
• DOI: 10.1093/jambio/lxae132

We investigated the effects of the aggregate spray-seeding (ASS) technique on soil bacterial community diversity, life strategies, and seasonal change. Soil from six plots with original vegetation (CK, n = 6) was compared to soil from 15 plots with spray-seeding restoration (SR, n = 15) using environmental DNA sequencing. The bacterial Shannon and Chao1 indices of SR soils were significantly greater (P < 0.05) than those of CK soils. The Chao1 index for the SR soil bacterial community was significantly greater in summer (P < 0.05) than in winter. The ratio of the relative abundance of bacterial K-strategists to r-strategists (K/r) and the DNA guanine-cytosine (GC) content in the SR soil were significantly lower (P < 0.05) than those in the CK soil. Principal coordinate analysis revealed significant differences between the SR and CK bacterial communities. The GC content was positively correlated with the K/r ratio. Soil conductivity was negatively associated with the K/r ratio and GC content, indicating that ionic nutrients were closely related to bacterial life strategies. The ASS technique improved soil bacterial diversity, altered community composition, and favored bacterial r-strategists.