Enumeration of CD4(+)CD25(+)T regulatory cells in Type-II diabetes retinopathy

• Published in: Pakistan journal of pharmaceutical sciences Vol. 27; no. 5; pp. 1191 - 1197
• Main Authors: Afzal, Nadeem, Javaid, Khursheed, Zaman, Shakeela, Zafar, Abu, Nagi, Abdul H
• Format: Journal Article
• Language: English
• Published: Pakistan 01.09.2014
• Subjects: Adult; Aged; Case-Control Studies; Cross-Sectional Studies; Diabetes Mellitus, Type 2 - immunology; Diabetic Retinopathy - immunology; Female; Flow Cytometry; Humans; Logistic Models; Lymphocyte Count; Male; Middle Aged; T-Lymphocytes, Regulatory - immunology
• ISSN: 1011-601X

Diabetes mellitus (DM) is a health concern because it leads to complications such as retinopathy. Pakistan has 6.9 million DM affected people that will be doubled by 2025. A study was designed to enumerate CD4(+)CD25(+)Treg cells in Pakistani type 2 diabetes mellitus (T2DM) patients. It was a cross-sectional case-control study that included 212 subjects. The subjects having diabetic retinopathy were labeled as Group-I (30 healthy volunteers without diabetes), Group-II (30 T2DM patients without retinopathy) and Group-III (152 T2DM patients with retinopathy). The percentage of CD4+CD25+ Treg cells was determined by Flowcytometry. Comparison of CD4(+)CD25(+)T cells among different groups was not significant and higher percentage of Treg cells was observed in Group-II (3.07%) compared to Group-III (2.88%). Age, gender and duration of diabetes may contribute while percentage of CD4(+)CD25(+)T cells and Treg cells were not associated with the development of DR in T2DM.