Letter: Evaluation and comparison of collision-induced dissociation and electron-capture dissociation for top-down analysis of intact ribonuclease B

• Published in: European journal of mass spectrometry (Chichester, England) Vol. 21; no. 4; p. 707
• Main Authors: Wang, Ze, Chen, Xiangfeng, Deng, Liulin, Li, Wan, Wong, Y-L Elaine, Chan, T-W Dominic
• Format: Journal Article
• Language: English
• Published: England 2015
• Subjects: Disulfides - chemistry; Electrons; Glycoproteins - chemistry; Ribonucleases - chemistry; Sequence Analysis, Protein - methods; Spectrometry, Mass, Electrospray Ionization - methods
• ISSN: 1469-0667
• DOI: 10.1255/ejms.1386

It has been previously reported that the glycosylation site and protein-sequence information could be obtained for ribonuclease B by top-down electron-capture dissociation (ECD) and collision-induced dissociation (CID) mass spectrometry (MS). However, the sequence coverage of ribonuclease B was limited in a single activation, and the structural information on the glycan moiety was not probed successfully in previous experiments. Here, we demonstrate that ECD and CID techniques can be used together as an effective top- down method for the structural characterization of intact glycoprotein. Even without an elaborate pre- or post- ECD activation, a high sequence coverage (<90%) of ribonuclease B could be achieved with substantial amounts of structural information for the glycan moiety. By comparing our work with previous results, it is postulated that the disulfide bond reduction strategy might play a significant role in determining the efficiency of top-down MS.