Gene expression of the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in the regulation of hematopoietic stem cell aging by ginsenoside Rg1

• Published in: Genetics and molecular research Vol. 13; no. 4; p. 10086
• Main Authors: Yue, Z, Rong, J, Ping, W, Bing, Y, Xin, Y, Feng, L D, Yaping, W
• Format: Journal Article
• Language: English
• Published: Brazil 04.12.2014
• Subjects: Animals; beta-Galactosidase - metabolism; Cellular Senescence - drug effects; Cellular Senescence - genetics; Cyclin-Dependent Kinase Inhibitor p16 - genetics; Cyclin-Dependent Kinase Inhibitor p16 - metabolism; Cyclin-Dependent Kinase Inhibitor p19 - genetics; Cyclin-Dependent Kinase Inhibitor p19 - metabolism; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Gene Expression Regulation - drug effects; Ginsenosides - chemistry; Ginsenosides - pharmacology; Hematopoietic Stem Cells - cytology; Hematopoietic Stem Cells - drug effects; Hematopoietic Stem Cells - metabolism; Mice, Inbred C57BL; Retinoblastoma Protein - genetics; Retinoblastoma Protein - metabolism; RNA, Messenger - genetics; RNA, Messenger - metabolism; Signal Transduction - drug effects; Signal Transduction - genetics; tert-Butylhydroperoxide - pharmacology; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - metabolism
• ISSN: 1676-5680; 1676-5680
• DOI: 10.4238/2014.December.4.3

The elucidation of the molecular mechanisms underlying the effects of traditional Chinese medicines in clinical practice is a key step toward their worldwide application, and this topic is currently a subject of intense research interest. Rg1, a component of ginsenoside, has recently been shown to perform several pharmacological functions; however, the underlying mechanisms of these effects remain unclear. In the present study, we investigated whether Rg1 has an anti-senescence effect on hematopoietic stem cells (HSCs) and the possible molecular mechanisms driving any effects. The results showed that Rg1 could effectively delay tert-butyl hydroperoxide (t-BHP)-induced senescence and inhibit gene expression in the p16(INK4a)-Rb and p19(Arf)-p53-p21(Cip/Waf1) signaling pathways in HSCs. Our study suggested that these two signaling pathways might be potential targets for elucidating the molecular mechanisms of the Rg1 anti-senescence effect.