Evaluation of interleukin-8 -251 t/a polymorphisms in visceral leishmaniasis

• Published in: Journal of research in health sciences Vol. 15; no. 1; pp. 59 - 61
• Main Authors: Hajilooi, Mehrdad, Abasi, Mohammad, Bazmani, Ahad, Ahmadi, Alireza, Matini, Mohammad, Solgi, Ghasem, Sardarian, Khosro
• Format: Journal Article
• Language: English
• Published: Iran 2015
• Subjects: Alleles; Cross-Sectional Studies; Gene Frequency; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Immunity - genetics; Interleukin-8 - genetics; Interleukin-8 - metabolism; Leishmania; Leishmaniasis, Visceral - genetics; Leishmaniasis, Visceral - immunology; Leishmaniasis, Visceral - parasitology; Polymorphism, Single Nucleotide; IL-8; Visceral leishmaniasis; Polymorphism
• ISSN: 2228-7809

Interleukin (IL)-8 plays important roles in the recruitment and activation of immune cells during visceral leishmaniasis (VL). Genetic variations in IL-8 modulate the expression of IL-8 protein and may be associated with VL. This study aimed to evaluate polymorphisms at the IL-8 -251 position in VL patients. This cross-sectional study was performed on three groups: Leishmania-seropositive patients with clinical symptoms of VL (n = 91), seropositive patients without clinical symptoms (n = 104), and healthy controls (n = 110). Polymorphisms at the IL-8-251 position were analyzed using allele-specific polymerase chain reaction (PCR). Anti-Leishmania antibody titers were assessed by immunofluorescence. IL-8-251 polymorphism was significantly associated with VL (P<0.002). The IL-8-251 T/T genotype was significantly higher in group 1 than in groups 2 and 3 (P<0.002). The validity of the data was analyzed using Hardy-Weinberg equilibrium and one-way analysis of variance (ANOVA), as well as χ2 tests. IL-8-251 polymorphism was significantly associated with impaired immune responses in VL and might be considered a risk factor for disease development.