Evidence of the circulation of hepatitis A virus, subgenotype IA, in environmental samples from Antioquia, Colombia

Hepatitis A virus (HAV) is an important pathogen, typically transmitted via the faecal-oral route. The epidemiology of the infection is directly related to drinking water access and adequate disposal of sewage water.  To determine the presence and identify the genotype of HAV in environmental sample...

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Published inBiomédica Vol. 36; pp. 135 - 147
Main Authors Báez, Paula Andrea, Jaramillo, Carlos Mario, Arismendi, Lina, Rendón, Julio Cesar, Cortés-Mancera, Fabián, Peláez, Dioselina, González, María Mercedes, Molina, Francisco, Navas, María Cristina
Format Journal Article
LanguageEnglish
Spanish
Published Colombia 16.02.2016
Subjects
Colombia - epidemiology
Environmental Microbiology
Genome, Viral - genetics
Genotype
Hepatitis A - epidemiology
Hepatitis A - virology
Hepatitis A virus - genetics
Humans
Phylogeny
RNA, Viral - genetics
phylogeny
Hepatitis viruses
drinking water
waste water
public health
genotype
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Summary:Hepatitis A virus (HAV) is an important pathogen, typically transmitted via the faecal-oral route. The epidemiology of the infection is directly related to drinking water access and adequate disposal of sewage water.  To determine the presence and identify the genotype of HAV in environmental samples from eight municipalities and two villages in Antioquia, northwestern Colombia.  Three serial samplings were done between December, 2012, and April, 2014. Water samples were obtained from drinking water plants prior to treatment, as well as from the main reserve of wastewater in each municipality included in the study. Viral concentrations for the two types of sample sources were determined by filtration/tangential ultrafiltration and polyethyleneglycol plus flocculation with skimmed milk, respectively. Total ARN was subsequently obtained from each sample and the VP3-VP1 region amplified for detection of the viral genome. The genotype was determined by amplification of the VP1-2B region.  The HAV genome was detected in samples from drinking water plants at Puerto Berrío, Frontino and Nutibara, and in wastewater samples from the municipalities of Arboletes, Zaragoza and Venecia. HAV subgenotype IA was identified using phylogenetic analysis.  In this study, HAV was identified in 6.6% of the samples from drinking water plants and 13.3% of wastewater samples. This is the first report of HAV subgenotype IA circulating in environmental samples from Antioquia.
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ISSN:0120-4157
DOI:10.7705/biomedica.v36i0.2979