Recognition of excretory/secretory antigens of Anisakis type I and evolution of IgE in experimentally infected rats

• Published in: Enfermedades infecciosas y microbiologia clinica Vol. 32; no. 8; p. 491
• Main Authors: Gómez-Mateos, Magdalena, Valero-López, Adela, de la Rubia-Nieto, Teresa, Romero-López, María Del Carmen, Díaz-Sáez, Victoriano
• Format: Journal Article
• Language: Spanish
• Published: Spain 01.10.2014
• Subjects: Allergens - immunology; Animals; Anisakiasis - immunology; Anisakis - immunology; Antibodies, Helminth - biosynthesis; Antibodies, Helminth - blood; Antibodies, Helminth - immunology; Antigens, Helminth - immunology; Calcium-Binding Proteins - immunology; Electrophoresis, Polyacrylamide Gel; Helminth Proteins - immunology; Immunoelectrophoresis; Immunoglobulin E - biosynthesis; Immunoglobulin E - immunology; Larva; Protein Denaturation; Rats; Rats, Wistar; Anisakiosis; Anisakis simplex spp; Immunoblotting; Anisakiasis; Inmunotransferencia
• ISSN: 1578-1852
• DOI: 10.1016/j.eimc.2013.09.009

Anisakis spp., during parasitism, release excretory-secretory antigens that, in contact with the human immune system, can trigger a hypersensitivity response mediated by IgE, causing various allergic symptoms. To evaluate the IgE response in Wistar rats after infection with L3 larvae of the parasite Anisakis spp. Some determining factors involved in the technique have been improved in this work, such as: the concentration of polyacrylamide used in the preparation of the gels, the antigen concentration used, and the temperature required for denaturation of proteins. Immune responses (Ag-Ab) observed by the immunoblotting technique showed a greater intensity with serum obtained after reinfection, which have recognized proteins that may correspond to the major antigen Ani s 1 and other polypeptides of interest in the diagnosis of human anisakiasis. This paper concludes that immunoblotting is a useful technique to detect IgE antibodies against Anisakis proteins.