Substituted aminophenols and flavonoids as potential components for test-systems for total antioxidant activity

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Vol. 2; no. 1; pp. 75 - 81
• Main Authors: Grigorenko, Yu. A., Karaseva, E. I., Metelitza, D. I., Sorokin, V. L., Ksendsova, G. A., Shadyro, O. I.
• Format: Journal Article
• Language: English
• Published: Moscow Nauka/Interperiodica 2008; Springer Nature B.V
• Subjects: Antioxidants; Biochemistry; Bioorganic Chemistry; Chemistry; Chemistry and Materials Science; Enzymes; Experimental Studies; Kinetics; Medicinal Chemistry; Oxidation; aminophenols; ortho-phenylenediamine; methemalbumin; pseudoperoxidase-catalyzed oxidation; flavonoids; antioxidant activity
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1007/s11828-008-1008-x

Kinetics of ortho -phenylenediamine (PDA) oxidation has been investigated in the “pseudoperoxidase” system, Methemalbumin-H 2 O 2 , in the presence of 2-amino-4- tert -butylphenol (ATBP), 2-amino-4,6-di tert -butylphenol (ADTBP) and its four N-acyl derivates, as well as flavonoids (quercetin, morin, silibin, hesperidin and naringin). Under standard experimental conditions (20°C, phosphate buffered saline, pH 7.4, containing 5.25% ethanol and DMF) ATBP, ADTBP and two its N-acyl-derivatives and also all five flavonoids inhibited PDA oxidation with different efficiency, which was characterized in terms of the inhibition constants, K i , M, or the percent of inhibition degree at the maximal concentrations of these inhibitors. Quercetin (K i = 7.7 × 10 −5 M) and ATBP (K i = 1.26 × 10 −4 M) were the most effective antioxidants. Using these characteristics and other required criteria, the pairs PDA-quercetin and PDA-ATBP were proposed for a practical application in the test-systems for evaluation of total antioxidant activity of biological objects.