Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs

• Published in: Journal of immunological methods Vol. 360; no. 1-2; pp. 96 - 102
• Main Authors: Bonnin, Brigitte, Correll, Anita, Swiatek-de Lange, Magdalena, Lenter, Martin, Schneider, Franz-Joseph, Wijdenes, John, Jonuleit, Helmut, Becker, Christian
• Format: Journal Article
• Language: English
• Published: Netherlands 31.08.2010
• Subjects: CD4 Antigens - biosynthesis; Cells, Cultured; Flow Cytometry; Forkhead Transcription Factors - biosynthesis; High-Throughput Screening Assays; Histocompatibility Antigens Class II - biosynthesis; Humans; Immune Tolerance; Immunomagnetic Separation; Integrin alpha4beta1 - biosynthesis; Interleukin-2 Receptor alpha Subunit - biosynthesis; Leukapheresis; T-Lymphocyte Subsets - immunology; T-Lymphocyte Subsets - metabolism; T-Lymphocyte Subsets - pathology; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - metabolism; T-Lymphocytes, Regulatory - pathology
• ISSN: 1872-7905
• DOI: 10.1016/j.jim.2010.06.014

The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research.