Large scale preparation of human MHC class II+ integrin beta(1)+ Tregs
The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isola...
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Published in | Journal of immunological methods Vol. 360; no. 1-2; pp. 96 - 102 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
31.08.2010
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Subjects | |
Online Access | Get full text |
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Summary: | The human CD4+CD25+FoxP3+ regulatory T cell population (Tregs) contains both MHC class II+ and MHC class II(-) cells. MHC class II+ Tregs belong to the integrin alpha(4)beta(1)+ subpopulation and exclusively execute contact-dependent suppressive activity. Here we present a method optimized for isolation of these MHC class II expressing Tregs from large leukaphereses products using magnetic microbeads that achieves a reproducible purity of more than 90% and enables the use of this small-sized Treg population in pre-clinical application and basic research. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1872-7905 |
DOI: | 10.1016/j.jim.2010.06.014 |