Spatial and Temporal Coordination of Force-generating Actin-based Modules Drives Membrane Remodeling In Vivo

Membrane remodeling drives a broad spectrum of cellular functions, and it is regulated through mechanical forces exerted on the membrane by cytoplasmic complexes. Here, we investigate how actin filaments dynamically tune their structure to control the active transfer of membranes between cellular co...

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Published inbioRxiv
Main Authors Heydecker, Marco, Shitara, Akiko, Chen, Desu, Tran, Duy, Masedunskas, Andrius, Tora, Muhibullah, Ebrahim, Seham, Appaduray, Mark A, Galeano Niño, Jorge Luis, Bhardwaj, Abhishek, Narayan, Kedar, Hardeman, Edna C, Gunning, Peter W, Weigert, Roberto
Format Journal Article
LanguageEnglish
Published United States 05.12.2023
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Summary:Membrane remodeling drives a broad spectrum of cellular functions, and it is regulated through mechanical forces exerted on the membrane by cytoplasmic complexes. Here, we investigate how actin filaments dynamically tune their structure to control the active transfer of membranes between cellular compartments with distinct compositions and biophysical properties. Using intravital subcellular microscopy in live rodents we show that: a lattice composed of linear filaments stabilizes the granule membrane after fusion with the plasma membrane; and a network of branched filaments linked to the membranes by Ezrin, a regulator of membrane tension, initiates and drives to completion the integration step. Our results highlight how the actin cytoskeleton tunes its structure to adapt to dynamic changes in the biophysical properties of membranes.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Working Paper/Pre-Print-1
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ISSN:2692-8205
2692-8205
DOI:10.1101/2023.12.04.569944