Isolation of respiratory syncytial virus from nasopharyngeal aspirates stored at 20 degrees C from one to fifteen months after collection

Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (...

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Published inMemórias do Instituto Oswaldo Cruz Vol. 101; no. 4; pp. 451 - 453
Main Authors Nunes, I F S, Moura, F E A
Format Journal Article
LanguageEnglish
Published Brazil 01.06.2006
Subjects
Cell Line
Cryopreservation - methods
Fluorescent Antibody Technique
Humans
Nasopharynx - virology
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Viruses - isolation & purification
Time Factors
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Summary:Cell culture isolation is used for recovering respiratory syncytial virus (RSV) from respiratory specimens. As RSV is a thermolabile virus, specimens destined for inoculation into cell culture require special transport, handling, and storage. The isolation rate of RSV from nasopharyngeal aspirates (NPA) stored at 20 20 degrees C for one to 15 months after collection was investigated. A total of 126 samples considered positive for RSV by indirect fluorescence-antibody were tested by virus isolation in HEp-2 cell culture. RSV was isolated from 47/126 specimens (37.3%). These results show that RSV may be recovered from NPA stored at 20 20 degrees C by cell culture.
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ISSN:0074-0276
1678-8060
DOI:10.1590/S0074-02762006000400017