Effect of diluents on motility of ram sperm during storage at 5 degrees C

• Published in: Archives of andrology Vol. 49; no. 1; pp. 69 - 75
• Main Authors: Gündoğan, M, Tekerli, M, Uçar, M, Türkmenoğlu, I
• Format: Journal Article
• Language: English
• Published: England 01.01.2003
• Subjects: Animals; Blood; Citric Acid - pharmacology; Egg Yolk; Glucosephosphates - pharmacology; Male; Milk; Organ Preservation Solutions - pharmacology; Seawater; Semen Preservation; Sheep; Sperm Motility - drug effects
• ISSN: 0148-5016
• DOI: 10.1080/01485010390129197

This study examined the effects of 5 diluents--glucose-phosphate (GP), skim milk-egg yolk (MY), egg yolk-citrate (YC), blood sera (S), and seawater (SW)--on daily motility of ram sperm stored at 5 degrees C for a short time. Ram semen collected by artificial vagina was diluted. Percentage of motile spermatozoa (PM) was assessed every 24 h up to day 10 after dilution. The different kinds of diluents except for SW had no effect on the primary motility of sperm and the primary motility was 0 with SW. There was a significant effect (p<.01) of diluent type and storage times in the conservation rates after cooling for PM. The GP diluent achieved greater values (80.5+/-0.88%) than S (69.5+/-1.70%) and MY (78.0+/-1.17%), while YC showed an intermediate value (79.0+/-1.24%). In relation to time of storage, GP and YC diluents showed the PM values up to day 10, but these values were 8 and 7 days for MY and S, respectively. The GP and YC extenders maintained a PM > 50% up to day 4, while MY was greater than 50% up to day 3 and S to day 2.