Immersion oil for high-resolution live-cell imaging at 37 degrees C: optical and physical characteristics

The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and in...

Full description

Saved in:
Bibliographic Details
Published inJournal of microscopy (Oxford) Vol. 232; no. 2; pp. 353 - 361
Main Authors Oomen, L C J M, Sacher, R, Brocks, H H J, Zwier, J M, Brakenhoff, G J, Jalink, K
Format Journal Article
LanguageEnglish
Published England 01.11.2008
Subjects
Animals
Cells, Cultured
Kidney - cytology
Mice
Microscopy, Confocal - methods
Microscopy, Fluorescence - methods
Oils
Temperature
Online AccessGet full text

Cover

More Information
Summary:The use of normal immersion oil, developed for 23 degrees C, at 37 degrees C greatly compromises both axial resolution and signal intensity. We developed and characterized an immersion oil for optimal performance in live-cell imaging at 37 degrees C. We quantify the improvements in resolution and intensity obtained when using the new oil instead of its standard 23 degrees C counterparts.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1365-2818
DOI:10.1111/j.1365-2818.2008.02106.x