CLA reduces antigen-induced histamine and PGE(2) release from sensitized guinea pig tracheae

• Published in: American journal of physiology. Regulatory, integrative and comparative physiology Vol. 280; no. 3; pp. R908 - R912
• Main Authors: Whigham, L D, Cook, E B, Stahl, J L, Saban, R, Bjorling, D E, Pariza, M W, Cook, M E
• Format: Journal Article
• Language: English
• Published: United States 01.03.2001
• Subjects: Animals; Antigens - immunology; Carbachol - pharmacology; Dietary Fats - administration & dosage; Dinoprostone - secretion; Eating; Female; Guinea Pigs; Histamine Release - drug effects; Hypersensitivity, Immediate - immunology; Hypersensitivity, Immediate - physiopathology; Linoleic Acid - administration & dosage; Linoleic Acid - analysis; Linoleic Acid - pharmacology; Muscle Contraction - drug effects; Ovalbumin - immunology; Trachea - chemistry; Trachea - immunology; Trachea - physiology; Weight Gain
• ISSN: 0363-6119
• DOI: 10.1152/ajpregu.2001.280.3.R908

Conjugated linoleic acid (CLA) has been shown to enhance immune reactions such as lymphocyte blastogenesis and delayed-type hypersensitivity. We investigated the role of CLA in type I (immediate) hypersensitivity, using a guinea pig tracheal superfusion model for measuring antigen-induced airway smooth muscle contraction and inflammatory mediator release. Female Hartley guinea pigs were fed a diet supplemented with 0.25 g corn oil or linoleic acid/100 g of diet (control) or 0.25 g CLA/100 g of diet for at least 1 wk before and during active sensitization to ovalbumin antigen. Tracheae from sensitized guinea pigs were suspended in air-filled water-jacketed (37 degrees C) tissue chambers in a superfusion apparatus. Tracheae were superfused with buffer containing antigen, and tissue contraction was recorded. Superfusate was collected at 90-s intervals for evaluation of histamine and PGE(2) release. CLA did not affect antigen-induced tracheal contractions when expressed as gram contraction per gram tissue. CLA significantly reduced antigen-induced histamine and PGE(2) release. CLA appears to decrease release of some inflammatory mediators during type I hypersensitivity reactions.