Skim milk enhances the preservation of thawed -80 degrees C bacterial stocks

The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory)....

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Bibliographic Details
Published inJournal of microbiological methods Vol. 75; no. 1; pp. 135 - 138
Main Authors Cody, William L, Wilson, James W, Hendrixson, David R, McIver, Kevin S, Hagman, Kayla E, Ott, C M, Nickerson, Cheryl A, Schurr, Michael J
Format Journal Article
LanguageEnglish
Published Netherlands 01.09.2008
Subjects
Animals
Bacteria - cytology
Bacteria - growth & development
Cryopreservation - methods
Cryoprotective Agents - pharmacology
Glycerol - pharmacology
Microbial Viability - drug effects
Milk
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Summary:The results from bacterial strain recovery efforts following hurricanes Katrina and Rita are reported. Over 90% of strains frozen in 10% skim milk were recovered whereas various recovery rates were observed for glycerol-stored stocks (56% and 94% of Escherichia coli, depending upon the laboratory). These observations led to a viability comparison of Streptococcus pyogenes, Campylobacter jejuni, Borrelia burgdorferi, Salmonella enterica subsp. Typhimurium, Pseudomonas aeruginosa and E. coli strains stored in glycerol or skim milk. In all bacteria examined, 10% skim milk resulted in significantly longer viability after thawing than 15% glycerol solutions currently used in most laboratories.
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ISSN:0167-7012
DOI:10.1016/j.mimet.2008.05.006