Oligobodies: bench made synthetic antibodies

Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "ol...

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Bibliographic Details
Published inMedicina (Buenos Aires) Vol. 59; no. 6; p. 753
Main Authors Radrizzani, M, Broccardo, M, González Solveyra, C, Bianchini, M, Reyes, G B, Cafferata, E G, Santa-Coloma, T A
Format Journal Article
LanguageEnglish
Published Argentina 1999
Subjects
Animals
Antibody Specificity
Blotting, Western
Immunohistochemistry
Mice
Mice, Inbred C57BL
Oligonucleotides - chemical synthesis
Oligonucleotides - immunology
Peptide Library
Phosphoprotein Phosphatases - immunology
Polymerase Chain Reaction
Protein Phosphatase 2
Rabbits
Sequence Analysis, DNA
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Summary:Using synthetic peptides and a combinatorial library of 56 mer random oligonucleotides, we have developed reagents that behave as "synthetic antibodies". The results obtained with the protein phosphatase 2A as a model system are shown here. The specificity of these reagents, named "oligobodies", has been demonstrated by Western blot analysis and immunohistochemistry. The oligobodies have enormous advantages compared to antibodies: their production is independent of the immune system, they can be prepared in a few days and there is no need for a purified target protein. These reagents can be produced even if the corresponding protein was never isolated or purified, since only a partial DNA sequence from a database provides enough information to make them.
ISSN:0025-7680