Secondary Raman spectrum of beta-carotene molecule in living leaf of French phoenix tree

Under visible incidence light 514.5 nm, the Raman scattering spectrum from the beta-carotene molecule in the leaf was directly obtained after it was immediately collected from French phoenix tree without any preparing the sample but cleaning. It is very easy to collect the secondary Raman lines addi...

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Bibliographic Details
Published inGuang pu xue yu guang pu fen xi Vol. 22; no. 5; p. 790
Main Authors Zhao, Jin-tao, Zhang, Peng-xiang, Xu, Cun-ying
Format Journal Article
LanguageChinese
Published China 01.10.2002
Subjects
beta Carotene - analysis
Magnoliopsida - chemistry
Photosynthesis
Plant Leaves - chemistry
Spectrum Analysis, Raman - methods
Trees - chemistry
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Summary:Under visible incidence light 514.5 nm, the Raman scattering spectrum from the beta-carotene molecule in the leaf was directly obtained after it was immediately collected from French phoenix tree without any preparing the sample but cleaning. It is very easy to collect the secondary Raman lines addition to the first Raman spectrum in situ by micro Raman. By careful comparing and analyzing the Raman lines between 2,000-3,100 cm-1 and below 2,000 cm-1 regions, we obtained the correlated relation of the first and secondary Raman lines. The study results indicated that there is no damage to the structure and configuration of beta-carotene molecule in the live leaf by controlling laser power on the sample surface and integrating time for Raman signal, but large power laser or long time irradiation on the live sample would cause very strong fluorescence background in Raman spectrum which indicated that there is a photo damage in the center of photo reaction. The Micro Raman would become one of possible in situ meth
ISSN:1000-0593