Interaction of nonelectrolytes, the derivatives of 5-hydroxybenzimidazole, with erythrocyte membrane

The method of spin probe and scanning electron microscopy were used to study the effects of some new synthetic antioxidants and bioregulators, the derivatives of 5-hydroxybenzimidazole, on the membrane structure and morphology of erythrocytes. Analysis of EPR spectra and electron micrographs reveale...

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Bibliographic Details
Published inBiofizika Vol. 50; no. 2; p. 310
Main Authors Luneva, O G, Gendel, L Ia, Kuznetsov, Iu V, Smirnov, L D
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.03.2005
Subjects
Animals
Antioxidants - pharmacology
Benzimidazoles - pharmacology
Cell Shape - drug effects
Electron Spin Resonance Spectroscopy
Erythrocyte Membrane - drug effects
Erythrocyte Membrane - ultrastructure
Erythrocytes - drug effects
Erythrocytes - ultrastructure
In Vitro Techniques
Male
Microscopy, Electron, Scanning
Rats
Spin Labels
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Summary:The method of spin probe and scanning electron microscopy were used to study the effects of some new synthetic antioxidants and bioregulators, the derivatives of 5-hydroxybenzimidazole, on the membrane structure and morphology of erythrocytes. Analysis of EPR spectra and electron micrographs revealed that the derivatives with various side substituents affect the membrane structure and shape of erythrocytes in a concentration-dependent manner, the effect correlating with the hydrophobic properties of the side derivatives. It was shown that all the compounds in the concentration range 1.10(-7) - 1.10(-3) M exhibit the echinocytogenic action, the most profound effect being found in the compound with benzyl- and ethoxygroup in sites 2 and 5, respectively. Our data suggest that nonelectrolytes, the derivatives of 5-hydroxybenzimidazole, are located in the outer monolayer of erythrocyte membrane.
ISSN:0006-3029