Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples

• Published in: Revista brasileira de parasitologia veterinaria Vol. 16; no. 1; pp. 5 - 9
• Main Authors: Nunes, Cáris M, Dias, Ana Karina K, Gottardi, Fernanda Patricia, De Paula, Henrique B, De Azevedo, Márcia A A, De Lima, Valéria M F, Garcia, José Fernando
• Format: Journal Article
• Language: Portuguese
• Published: Brazil 01.01.2007
• Subjects: Animals; DNA, Protozoan - analysis; Dog Diseases - blood; Dog Diseases - diagnosis; Dog Diseases - parasitology; Dogs; Leishmania - genetics; Leishmaniasis, Visceral - diagnosis; Leishmaniasis, Visceral - veterinary; Polymerase Chain Reaction

Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood s...