Polymerase chain reaction evaluation for canine visceral leishmaniasis diagnosis in dog blood samples

Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood s...

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Published inRevista brasileira de parasitologia veterinaria Vol. 16; no. 1; pp. 5 - 9
Main Authors Nunes, Cáris M, Dias, Ana Karina K, Gottardi, Fernanda Patricia, De Paula, Henrique B, De Azevedo, Márcia A A, De Lima, Valéria M F, Garcia, José Fernando
Format Journal Article
LanguagePortuguese
Published Brazil 01.01.2007
Subjects
Animals
DNA, Protozoan - analysis
Dog Diseases - blood
Dog Diseases - diagnosis
Dog Diseases - parasitology
Dogs
Leishmania - genetics
Leishmaniasis, Visceral - diagnosis
Leishmaniasis, Visceral - veterinary
Polymerase Chain Reaction
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Summary:Canine and human visceral leishmaniasis is endemic in several States of Brazil, and it is associated with infected dogs and the presence of the vector. Aiming at using polymerase chain reaction as a diagnostic tool in dogs, we amplified a 120bp fragment from kDNA of Leishmania spp. by PCR in blood samples. The lower detection limit observed was 0.1 parasites per 500microL of blood, which is a highly satisfactory result. On the other hand, PCR evaluation in 166 blood samples of dogs from Poxoréo, MS, Brazil, resulted in 55% sensitivity and 66.3% specificity, considering indirect imunnofluorescent test as gold standard.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0103-846X