Interaction of the Latrodectus tredecimguttatus spider venom with liposomes

It is established that interaction of Latrodectus tredecimguttatus venom with bilayer phospholipid membranes of liposomes results in formation of ion channels. Liposome permeability variations are estimated by input and output of 86Rb+ and 45Ca2+ isotopes. It is shown that LaCl3 in 0.5 mM concentrat...

Full description

Saved in:
Bibliographic Details
Published inUkrainskij biohimičeskij žurnal Vol. 57; no. 3; p. 7
Main Authors Trikash, I O, Lishko, V K
Format Journal Article
LanguageRussian
Published Ukraine 01.05.1985
Subjects
Animals
Arthropod Venoms - pharmacology
Black Widow Spider
Chromatography, Gel
Electrophoresis, Polyacrylamide Gel
In Vitro Techniques
Ion Channels - drug effects
Liposomes - metabolism
Membrane Lipids - metabolism
Permeability
Spider Venoms - metabolism
Spider Venoms - pharmacology
Spiders
Online AccessGet more information

Cover

More Information
Summary:It is established that interaction of Latrodectus tredecimguttatus venom with bilayer phospholipid membranes of liposomes results in formation of ion channels. Liposome permeability variations are estimated by input and output of 86Rb+ and 45Ca2+ isotopes. It is shown that LaCl3 in 0.5 mM concentration decreases considerably both input and output of Rb+ in proteoliposomes and it may be used as an inhibitor of latrotoxin channels in experiments with phospholipid vesicles. When studying electrophoretic spectrum of proteins bound with liposomes it is shown that latrotoxin (a polypeptide) with molecular mass 130 kDa predominates in proteoliposomes.
ISSN:0201-8470