High velocity mechanical injection of foreign DNA into fish oocytes

High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died...

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Bibliographic Details
Published inGenetika Vol. 26; no. 12; p. 2122
Main Authors Kolesnikov, V A, Alimov, A A, Barmintsev, V A, Beniumov, A O, Zelenina, I A, Krasnov, A M, Dzhabur, R, Zelenin, A V
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.12.1990
Subjects
Animals
beta-Galactosidase - genetics
Cypriniformes - genetics
DNA - administration & dosage
Kanamycin Kinase
Microinjections
Oocytes - metabolism
Phosphotransferases - genetics
Trout - genetics
Tungsten
Zebrafish - genetics
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Summary:High-velocity tungsten microprojectiles were used to introduce into fertilized eggs of loach (Misgurnus fossilis), Rainbow trout (Salmo gairdneri Rich.) and Zebra fish (Brachydanio rerio) DNA sequences of beta-galactosidase and neomycin phosphotransferase genes. No more than 30% of fish oocytes died as a result of bombardment. Experiments revealed marked activity of both enzymes in developing fishes. Neo gene DNA sequences were found in total danio DNA using PCR technique.
ISSN:0016-6758