Kinetic data of cytoplasmic estradiol receptor

Association and dissociation equilibrium constants and rate constants have been determined in human breast cancer tissues, human lymph node metastases, experimentally induced rat mammary tumors and pooled uteri of normal rats. When the dissociation constants gained from the Scatchard plot applying t...

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Bibliographic Details
Published inEndocrinologia experimentalis Vol. 17; no. 1; p. 23
Main Authors Görlich, M, Kleitke, C, Heise, E
Format Journal Article
LanguageEnglish
Published Slovakia 01.03.1983
Subjects
9,10-Dimethyl-1,2-benzanthracene
Animals
Binding Sites
Breast Neoplasms - metabolism
Cytoplasm - metabolism
Cytosol - metabolism
Estradiol - metabolism
Female
Humans
Kinetics
Lymphoma - metabolism
Lymphoma - secondary
Mammary Neoplasms, Experimental - metabolism
Mathematics
Rats
Rats, Inbred Strains
Receptors, Estradiol
Receptors, Estrogen - metabolism
Uterus - metabolism
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Summary:Association and dissociation equilibrium constants and rate constants have been determined in human breast cancer tissues, human lymph node metastases, experimentally induced rat mammary tumors and pooled uteri of normal rats. When the dissociation constants gained from the Scatchard plot applying the Chamness correction were compared to those calculated as the quotient of the rate constants, relatively large differences were found, predominantly due to the values of measured association rate constants. In contrast, there were no specific differences between the constants of distinct tissues. The differences among the association rate constants were presumably caused by a contamination of cytosols by steroid binding proteins of serum. Increasing amounts of serum added to tissue samples resulted in an increasing interference with the determination of the dissociation constants using the Scatchard analysis.
ISSN:0013-7200