Structural properties of leukemic and normal lymphoid cells

DNA synthesis intensity and spectral and fluorescent properties of leucemic, PHA-induced and intact normal mouse spleen cells and of nuclei isolated from these cells were investigated. The cell electrophoretic mobility and DNA-protein interaction in the nuclei were studied. Similarity in cell and nu...

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Bibliographic Details
Published inBiofizika Vol. 31; no. 4; p. 638
Main Authors Svirnovskiĭ, A I, Tsygankov, V G, Medved', I N, Shimanskaia, T V, Levin, V I
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1986
Subjects
Animals
Cells, Cultured
DNA - biosynthesis
DNA, Neoplasm - biosynthesis
Fluorescent Dyes
Leukemia, Experimental - metabolism
Lymphocyte Activation
Lymphocytes - metabolism
Mice
Mice, Inbred C57BL
Spectrophotometry, Ultraviolet
Spleen - cytology
Spleen - metabolism
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Summary:DNA synthesis intensity and spectral and fluorescent properties of leucemic, PHA-induced and intact normal mouse spleen cells and of nuclei isolated from these cells were investigated. The cell electrophoretic mobility and DNA-protein interaction in the nuclei were studied. Similarity in cell and nuclei fluorescence, fluorescence of the probe ANS conjugated with the cells, the electrophoretic mobility and tightness of DNA--protein interaction for leucemic and PHA--induced cells and also the similarity of the tightness of DNA--protein interaction for leucemic and normal intact cells were found inspite of the differences in DNA synthesis intensity and cell functional peculiarities.
ISSN:0006-3029