In vivo study of the antilipoperoxidant effect of 3',5,7-trihydroxy-4'-methoxy flavone 7 rutinoside

• Published in: Annales de biologie clinique (Paris) Vol. 52; no. 3; p. 171
• Main Authors: Freneix-Clerc, M, Dumon, M F, Carbonneau, M A, Thomas, M J, Peuchant, E, Dubourg, L, Melin, A M, Perromat, A, Clerc, M
• Format: Journal Article
• Language: French
• Published: France 1994
• Subjects: Acute-Phase Reaction - blood; Acute-Phase Reaction - chemically induced; Animals; Carbon Tetrachloride - pharmacology; Carrageenan - pharmacology; Diosmin - administration & dosage; Diosmin - pharmacology; Injections, Intraperitoneal; Lipid Peroxides - blood; Male; Rats; Rats, Wistar
• ISSN: 0003-3898; 1950-6112

In 3-month-old Wistar rats carrageenan and CCl4 injected intraperitoneally induce an acute phase reaction which is characterized by a marked increase in alpha 1, alpha 2, beta serum globulins. This reaction corresponds to a large increase in these globulins in the first case and a smaller one in the second. A lipoperoxidant effect is demonstrated by the serum lipoprotein mobility as the lipoperoxidation index (in MDA units) or the decrease in serum vitamin A and E concentrations. This effect is also greater in the first case than in the second one. In the same way the lipoperoxidant effect is shown in liver microsomes but with a lower amplitude in the first case than in the second one. The treatment of rats by intraperitoneal injection of diosmine (150 mg/kg per week) during the 8 weeks which precede the injection of carrageenan or CCl4 results in: i) a marked decrease in the acute-phase reaction and a lower one in the lipoperoxidant effect, in serum; ii) a decrease in the CCl4 induced lipoperoxidant effect in liver microsomes. It may be concluded that diosmine, not injected at the same time as carrageenan or CCl4, but during the previous 8 weeks is sufficiently well distributed in the whole body to produce a marked inhibition of the acute phase reaction and a perceptible effect on lipoperoxidation. It may be considered an effective complement to the natural antioxidant defences of the organism (vitamins A and E).