Comparative data on the propagation of cells from commercial common and leukemia-free chick embryos

Certain differences were found in comparative studies of optimal conditions for preparation and propagation of cell cultures from common and leukemia-free chick embryos. The yield of cells per 1 g of tissue was regularly higher by 12-20% with leukemia-free chick embryos than with common chick embryo...

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Bibliographic Details
Published inVoprosy virusologiĭ no. 4; p. 493
Main Author Ermakova, M N
Format Journal Article
LanguageRussian
Published Russia (Federation) 01.07.1976
Subjects
Animals
Cell Division
Chick Embryo
Culture Media
Culture Techniques
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Summary:Certain differences were found in comparative studies of optimal conditions for preparation and propagation of cell cultures from common and leukemia-free chick embryos. The yield of cells per 1 g of tissue was regularly higher by 12-20% with leukemia-free chick embryos than with common chick embryos. The viability of the latter under conditions of roller cultivation was lower. Monolayer cultures of cells from leukemia-free chick embryos formed 1 day earlier than those from cells of common chick embryos. The optimal medium for growth of leukemia-free chick embryo cells was 0.5% lactalbumin hydrolysate, and for common chick embryo cells--Eagle's medium.
ISSN:0507-4088