Strategies for improving heterologous protein production from filamentous fungi

Despite the naturally high capacity for protein secretion by many species of filamentous fungi, secreted yields of many heterologous proteins have been comparatively low. The strategies for yield improvement have included the use of strong homologous promoters, increased gene copy number, gene fusio...

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Bibliographic Details
Published inAntonie van Leeuwenhoek Vol. 65; no. 3; p. 245
Main Authors Archer, D B, Jeenes, D J, Mackenzie, D A
Format Journal Article
LanguageEnglish
Published Netherlands 1994
Subjects
Animals
Aspergillus niger - genetics
Aspergillus niger - metabolism
Fungi - genetics
Fungi - metabolism
Glucan 1,4-alpha-Glucosidase - biosynthesis
Glucan 1,4-alpha-Glucosidase - genetics
Muramidase - biosynthesis
Muramidase - genetics
Mycology - methods
Protein Processing, Post-Translational
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Transcription, Genetic
Transformation, Genetic
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Summary:Despite the naturally high capacity for protein secretion by many species of filamentous fungi, secreted yields of many heterologous proteins have been comparatively low. The strategies for yield improvement have included the use of strong homologous promoters, increased gene copy number, gene fusions with a gene encoding a naturally well-secreted protein, protease-deficient host strains and screening for high yields following random mutagenesis. Such approaches have been effective with some target heterologous proteins but not others. Approaches used in heterologous protein production from filamentous fungi are discussed and a perspective on emerging strategies is presented.
ISSN:0003-6072
DOI:10.1007/BF00871952