Effects of dihydrotestosterone, 17 beta-estradiol and dexamethasone on mitogen--and allogeneic--induced lymphoblastogenesis

In contrast to the in vivo action of sex steroids the steroidal hormones dihydrotestosterone (DHT) and estradiol (E2) produced a marked variable response on mitogen-induced lymphoblastogenesis. DHT and E2 at concentrations of 10(-11) mol l-1 incubated with mononuclear cell fraction in the presence o...

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Bibliographic Details
Published inEndocrinologia experimentalis Vol. 17; no. 1; p. 69
Main Authors Ow, K T, Abandowitz, H M, Sarfaty, G A, Nash, A R
Format Journal Article
LanguageEnglish
Published Slovakia 01.03.1983
Subjects
Adult
Concanavalin A - pharmacology
Dexamethasone - pharmacology
Dihydrotestosterone - pharmacology
Estradiol - pharmacology
Female
Humans
Lymphocyte Activation - drug effects
Lymphocyte Culture Test, Mixed
Lymphocytes - cytology
Lymphocytes - drug effects
Male
Mitogens - pharmacology
Mitosis - drug effects
Phytohemagglutinins - pharmacology
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Summary:In contrast to the in vivo action of sex steroids the steroidal hormones dihydrotestosterone (DHT) and estradiol (E2) produced a marked variable response on mitogen-induced lymphoblastogenesis. DHT and E2 at concentrations of 10(-11) mol l-1 incubated with mononuclear cell fraction in the presence of mitogens PHA, Con A and PWM caused inhibition, stimulation or no effect. Separation of T and B cell fractions and incubation with DHT and E2 likewise produced variations in response. However, dexamethasone (DEX) strongly inhibited proliferation of all cell fractions, particularly at the higher steroid concentrations. These results suggest that the reported effects of in vivo administered sex hormones on the immune system might possibly be mediated by a precursor cell of peripheral blood mononuclear cells, and that the in vitro system may not be a reproducible test for determining an individual's immunoendocrine status.
ISSN:0013-7200