Murine transforming growth factor-beta 2 cDNA sequence and expression in adult tissues and embryos

• Published in: Molecular endocrinology (Baltimore, Md.) Vol. 3; no. 7; pp. 1108 - 1114
• Main Authors: Miller, D A, Lee, A, Pelton, R W, Chen, E Y, Moses, H L, Derynck, R
• Format: Journal Article
• Language: English
• Published: United States 01.07.1989
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Blotting, Northern; DNA Probes - isolation & purification; Embryonic and Fetal Development; Fetus - metabolism; Humans; Mice; Molecular Sequence Data; Protein Precursors; Proteins - metabolism; RNA, Messenger - metabolism; Sequence Homology, Nucleic Acid; Transforming Growth Factor beta; Transforming Growth Factors - metabolism
• ISSN: 0888-8809
• DOI: 10.1210/mend-3-7-1108

Murine transforming growth factor-beta 2 (TGF-beta 2) cDNAs were isolated from cDNA libraries derived from a differentiated murine embryonic carcinoma cell line, PCC3. The composite cDNA sequence is 4267 nucleotides long, including a 1217 nucleotides 5'-untranslated sequence, and encodes a murine TGF-beta 2 precursor of 414 amino acids with 96% identity to its human counterpart. Several consensus polyadenylation sequences are present in the 1807 nucleotides 3'-untranslated sequence. Five TGF-beta 2 mRNA species are observed in the developing mouse fetus and they show different patterns of expression during development. TGF-beta 2 mRNA expression was also examined in adult mouse tissues, in which four of the five RNA species were observed. TGF-beta 2 mRNAs were present in all adult mouse tissues examined, except liver, and was most abundant in placenta, the male submaxillary gland and lung. The patterns of expression suggest a physiological role for TGF-beta 2 both in embryonic development and in the maintenance of adult tissues.