Observing the deoxy myoglobin and hemoglobin signals from rat myocardium in situ

1H NMR proximal histidyl NdeltaH signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O2, the...

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Published inFEBS letters Vol. 434; no. 3; pp. 309 - 312
Main Authors Tran, T K, Kreutzer, U, Jue, T
Format Journal Article
LanguageEnglish
Published England 04.09.1998
Subjects
Animals
Hemoglobins - metabolism
Male
Myocardium - metabolism
Myoglobin - analogs & derivatives
Myoglobin - metabolism
Rats
Rats, Sprague-Dawley
Signal Transduction
Temperature
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Summary:1H NMR proximal histidyl NdeltaH signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O2, the erythrocyte Hb is partially desaturated and yields the alpha and beta proximal histidyl NdeltaH signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO2.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0014-5793
DOI:10.1016/S0014-5793(98)01001-1