Active enhancement of canine liver allograft by pretreatment with polyspecific spleen alloantigen (author's transl)

• Published in: Langenbecks Archiv für Chirurgie Vol. 344; no. 1; p. 15
• Main Authors: Lie, T S, Holst, A, Kanda, M, Oehr, P, Rau, G S, Biersack, H J, Rommelsheim, K, Grünn, U
• Format: Journal Article
• Language: German
• Published: Germany 13.07.1977
• Subjects: Animals; Cadaver; Cell Migration Inhibition; Dogs; Epitopes; Graft Rejection; Immunologic Techniques; Isoantigens - administration & dosage; Isoantigens - isolation & purification; Liver Transplantation; Preoperative Care; Spleen - immunology; Time Factors; Transplantation Immunology; Transplantation, Homologous
• ISSN: 0023-8236
• DOI: 10.1007/BF01259349

Because it is not possible to use donor specific antigens for the induction of immunological enhancement in cadaveric organtransplantation, attempts were made to use polyspecific antigens in the enhancement of orthotopic canine liver allotransplants. Of 34 mongrel recipients, 17 controls survived for 6.9 +/- 1.5 days. Six recipients (group 3) were given 750 mg/kg polyspecific, semisoluble antigen prepared from 20 spleens (PSEA 20) together with 10 mg/kg prednisolone on days 15, 8 and 1 before transplantation. The mean survival time of this group was 10.1 +/- 2.0 days (P less than 0.01 compared with the control group). Six other recipients (group 4) were treated similiarly, except that the antigen had been prepared from a pool of 70 spleens (PSEA 70). Survival was variable here: 3 survived for more than 3 weeks and the other 3 died on days 1, 7 and 8 post-operatively, with signs of accelerated rejection. Donors and recipients were not identical for LD determinants, but one donor recipient pair with near identity showed a higher degree of enhancement. The recipients displaying accelerated rejection had markedly higher lymphocytotoxic and haemagglutinating antibodies. Animals surviving for longer periods had low antibody titres. In addition, all recipients progressive rejection were found to show inhibition of leucocyte migration. After three antigen doses rosette-forming lymphocytes were present in increased numbers in peripheral blood, and remained unchanged thereafter.