Isolation and maintenance of Batrachochytrium salamandrivorans cultures

Abstract Discovered in 2013, Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, Batrachochytrium dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal po...

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Published inbioRxiv
Main Authors Robinson, Kristyn A, Pereira, Kenzie E, Bletz, Molly C, Edward Davis Carter, Gray, Matthew J, Piovia-Scott, Jonah, Romansic, John M, Woodhams, Douglas C, Fritz-Laylin, Lillian
Format Paper
LanguageEnglish
Published Cold Spring Harbor Cold Spring Harbor Laboratory Press 08.10.2019
Subjects
Batrachochytrium salamandrivorans
Biodiversity
Pathogens
Skin diseases
Skin lesions
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Summary:Abstract Discovered in 2013, Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, Batrachochytrium dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a media for optimal Bsal growth, standard culture protocols, and a method for isolating Bsal from infected tissue.
DOI:10.1101/796920