Effects of acupotomy therapy on macrophage polarization and inhibition of synovial inflammation in rabbits with knee osteoarthritis based on the Notch1/Jagged1/Hes1 signaling pathway

• Published in: Zhēncì yánjiū Vol. 50; no. 6; p. 649
• Main Authors: Yu, Wen-Ying, Xiu, Zhong-Biao, Lin, Ze-Hao, Liu, Hong, Zhang, Liang-Zhi, Li, Yu-Lin, Feng, Xiang-Long, Liu, Jing
• Format: Journal Article
• Language: Chinese
• Published: China 25.06.2025
• Subjects: Acupuncture Points; Acupuncture Therapy; Animals; Humans; Jagged-1 Protein - genetics; Jagged-1 Protein - immunology; Jagged-1 Protein - metabolism; Macrophages - cytology; Macrophages - immunology; Male; Osteoarthritis, Knee - genetics; Osteoarthritis, Knee - immunology; Osteoarthritis, Knee - metabolism; Osteoarthritis, Knee - therapy; Rabbits; Receptor, Notch1 - genetics; Receptor, Notch1 - immunology; Receptor, Notch1 - metabolism; Signal Transduction; Transcription Factor HES-1 - genetics; Transcription Factor HES-1 - immunology; Transcription Factor HES-1 - metabolism; Notch1/Jagged1/Hes1 signaling pathway; Inflammation; Knee osteoarthritis; Macrophage polarization; Acupotomy
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.20240962

To observe the effects of acupotomy therapy on synovial inflammation, macrophage polarization, and protein expressions of drosophila double-wing edge notch homologous gene 1 (Notch1)/Jagged typical Notch ligand 1 (Jagged1)/Hes family BHLH transcription factor 1 (Hes1) signaling pathway, so as to explore its mechanisms underlying improvement of knee osteoarthritis (KOA). Male New Zealand rabbits were randomly divided into the blank, model and acupotomy groups (8 rabbits in each group). The KOA model was established by immobilizing the left hind limb in an extended position using a modified Videman method with a plaster cast for 6 weeks. After modeling, 3 meridian lesion points with significant reactions were selected from "Hedingci", "Binwaishang" "Binneishang" "Chengfeijian" "Weiyangci" and "Yinlingci" for acupotomy treatment, once a week for a total of 4 times. X-ray detection was performed after modeling. Behavioral changes in the affected knee joint of the rabbits were observed before and after the interve