Investigation of the Effects of Arsenic Trioxide Combined with Deslorelin on Proliferation and Apoptosis of Jurkat Cells Based on Wnt/β-Catenin Pathway

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 33; no. 3; p. 640
• Main Authors: Liu, Wei-Wan, Ren, Cui-Ai
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2025
• Subjects: Apoptosis - drug effects; Arsenic Trioxide; Arsenicals - pharmacology; beta Catenin - metabolism; Bridged Bicyclo Compounds, Heterocyclic - pharmacology; Cell Proliferation - drug effects; Humans; Jurkat Cells; Oxides - pharmacology; Wnt Signaling Pathway - drug effects; arsenic trioxide; NCTD; Jurkat cells; Wnt/β-catenin signaling pathway; apoptosis
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2025.03.003

To investigate the effect of Arsenic trioxide (ATO) combined with Norcantharidin (NCTD) on the proliferation and apoptosis of Jurkat cells, and to evaluate its effect on the proliferation and apoptosis of acute T-lymphoblastic leukemia (T-ALL) based on the Wnt/β-catenin signaling pathway. Jurkat cell lines were used as the study subjects and treated with different concentrations of ATO (0, 2, 4, 8, 16 μmol/L) and NCTD (0, 10, 25, 50, 100 μmol/L) for 72 hours, and the cell proliferation was detected by CCK-8. Meanwhile, flow cytometry was used to detect the apoptosis rate, EdU staining to detect cell proliferation viability, cell clone formation assay to assess cell cloning ability, Transwell assay to assess cell invasion ability, and Western blot to detect apoptosis and the expression of Wnt/β-catenin signaling pathway-related proteins. Compared with the control group, both ATO and NCTD effectively inhibited Jurkat cell proliferation when used alone, and the inhibition effect was more significant when used in