Topical application of vascular endothelial growth factor and bone marrow mesenchymal stem cell caused effects to the survival rate of random flap

Experimental study on the survival rate of random flap using vascular endothelial growth factor (VEGF) and bone marrow mesenchymal stem cell (MSC). MSC was isolated through the combined utilization of density gradient centrifugation and adherence screening methods. MSC was cultured and its purity an...

Full description

Saved in:
Bibliographic Details
Published inHua xi kou qiang yi xue za zhi Vol. 30; no. 6; p. 645
Main Authors Liu, Chunli, Liu, Shuhui, Liu, Zhihui
Format Journal Article
LanguageChinese
Published China 01.12.2012
Subjects
Animals
Bone Marrow Cells
Cell Differentiation
Humans
Rats
Rats, Sprague-Dawley
Rats, Wistar
Surgical Flaps
Survival Rate
Vascular Endothelial Growth Factor A
Online AccessGet more information

Cover

More Information
Summary:Experimental study on the survival rate of random flap using vascular endothelial growth factor (VEGF) and bone marrow mesenchymal stem cell (MSC). MSC was isolated through the combined utilization of density gradient centrifugation and adherence screening methods. MSC was cultured and its purity and biological properties were identified. Forty Wistar rat models were established and divided into control group, VEGF group, MSC group and MSC+VEGF group. Designed 2 cm x 5 cm pedicle flap on each rat's back. The flaps were observed for 14 d after operation. On the 14th day, the rats were killed. The survival rates of random flap in each group were counted, and the histological sections were stained at the flap which was 4 cm far from the pedicle, and the number of microvascular was observed. 1) MSC was detected by flow cytometry, adipogenic and osteogenic induction detection. The results showed that MSC could differentiate to fat and cartilage by induction and certified that the MSC had multi-differentiation pote
ISSN:1000-1182