Screening and Identification of lncRNA Related to Adipocity of Bone Marrow Microenvironment in Aplastic Anemia

• Published in: Zhongguo shi yan xue ye xue za zhi Vol. 32; no. 2; p. 610
• Main Authors: Liu, Leg, Zhang, Huan-Huan, Zhang, Xian-Ning, Liu, Lu-Lu, Chen, Ming-Tai
• Format: Journal Article
• Language: Chinese
• Published: China 01.04.2024
• Subjects: Adipogenesis; Adiposity; Anemia, Aplastic - genetics; Bone Marrow - metabolism; Bone Marrow Cells; CCAAT-Enhancer-Binding Protein-alpha - genetics; Cell Differentiation; Humans; Mesenchymal Stem Cells - metabolism; PPAR gamma - genetics; PPAR gamma - metabolism; RNA, Long Noncoding - genetics; aplastic anemia; bone marrow mesenchymal stem cell; PPARγ; C/EBPα; long noncoding RNA
• ISSN: 1009-2137
• DOI: 10.19746/j.cnki.issn.1009-2137.2024.02.043

To systematically screen and identify long noncoding RNA (lncRNA) associated with bone marrow adiposity changes in aplastic anemia (AA). The PPARγ and C/EBPα ChIP-Seq data in ChIPBase was analyzed by bioinformatics and the potential lncRNA co-transcriptionally regulated by PPARγ and C/EBPα was screened. The expression of candidate lncRNA was verified by qRT-PCR in the adipogenic differentiation model of BM-MSC, BM-MSC infected with lenti-shPPARγ and lenti-shC/EBPα as well as clinical BM-MSC samples derived from AA and controls. and were significantly highly expressed in AA BM-MSC, and knock-down of and impaired the adipogenic capacity of AA BM-MSC. PPARγ and C/EBPα cotranscriptionally activate promoter activity in binding sites dependant manner. The was also aberrantly highly expressed in AA BM-MSC compared with controls. and are aberrantly expressed in AA BM-MSC and may promote the adipogenic differentiation of AA BM-MSC, and to a certain extent mediate the bone marrow adiposity alteration by transcriptional