Protection of huanglian jiedu decoction on systemic and vascular immune responses of high fat induced apoE(-/-) mice

• Published in: Zhongguo Zhong xi yi jie he za zhi Vol. 33; no. 11; p. 1520
• Main Authors: Ma, Ya-luan, Wang, Bei-bei, Han, Jun-yan, Li, Rui, Zhang, Wei-mei, Li, Tong, Chen, Bing, Su, Jing, Wang, Xian-bo, Zeng, Hui
• Format: Journal Article
• Language: Chinese
• Published: China 01.11.2013
• Subjects: Animals; Aorta - pathology; Apolipoproteins E - genetics; CD36 Antigens - metabolism; Chemokine CCL2 - metabolism; Dietary Fats - adverse effects; Drugs, Chinese Herbal - pharmacology; Female; Hyperlipidemias - blood; Hyperlipidemias - etiology; Hyperlipidemias - immunology; Inflammation; Interleukin-10 - blood; Interleukin-12 - blood; Interleukin-1beta - blood; Leukocytes, Mononuclear - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; Systemic Inflammatory Response Syndrome - blood; Systemic Inflammatory Response Syndrome - etiology; Systemic Inflammatory Response Syndrome - immunology; Toll-Like Receptor 4 - metabolism; Transforming Growth Factor beta - blood; Tumor Necrosis Factor-alpha - blood
• ISSN: 1003-5370

To observe the effect of Huanglian Jiedu IJecoction (HJU) on systemic and vascular immune responses of high fat diet fed apoE deficient (apoE(-/-)) mice. Eight wild type C57BL6 mice were recruited as the wild type common food group. Totally 24 apoE(-/-) mice were randomly divided into the ApoE'common food group, the ApoE(-/-) hyperlipidemia group, and the ApoE(-/-) hyperlipidemia plus HJD group, 8 in each group. In the present study, the common food mice and high fat fed mice were fed with a chow diet or a high cholesterol diet for 4 weeks. HJD was given to mice in the ApoE(-/-) hyperlipidemia plus HJD group at the daily dose of 5 g/kg by gastrogavage, while equal volume of pure water was given to mice in the rest groups by gastrogavage. Four weeks later, the plasma levels of blood lipids, the ratio of peripheral blood mononuclear cells, and expressions of Toll-like receptor 4 (TLR-4) and CD36 on the monocytes were detected. The pathological changes and expressions of cytokines in local aorta were detected. T