Electroacupuncture preconditioning relieves cutaneous passive anaphylaxis by down-regulating IP3 mediated ROS/TRPM2 signaling pathway and inhibiting mast cell degranulation in rats with urticaria

• Published in: Zhēncì yánjiū Vol. 48; no. 3; p. 274
• Main Authors: Li, Ji-Quan, Li, Si-Jia, Wang, Lie, Chen, Yi-Ran, Wang, Ying, Liu, Jing-Jing, Dai, Jian-Yu, Wang, Wei, Ma, Tie-Ming
• Format: Journal Article
• Language: Chinese
• Published: China 25.03.2023
• Subjects: Anaphylaxis; Animals; Cell Degranulation; Electroacupuncture; Male; Mast Cells; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Signal Transduction; TRPM Cation Channels - genetics; Urticaria; Mast cells; Calcium ion; Electroacupuncture; Transient receptor potential channel; Urticaria
• ISSN: 1000-0607
• DOI: 10.13702/j.1000-0607.20220166

To observe the effect of electroacupuncture (EA) preconditioning of "Quchi" (LI11) and "Xuehai" (SP10) on mast cell (MC) degranulation, and expressions of inositol triphosphate(IP3), reactive oxygen species (ROS), transient receptor potential (TRP) M2, calmodulin (CaM) in rats with urticaria, so as to reveal its molecular mechanism under-lying improving urticaria. Thirty-two male SD rats were randomly divided into blank control, model, preconditioning of EA (Pre-EA) and medication groups ( =8 rats/group). The urticaria model was established by intradermal injection of dilute allogeneic antioalbumin serum at the spots of the bilateral symmetry of the spine on the back, and followed by tail venous injection of mixture solution of egg albumin diluent, plus 0.5% Evans blue and normal saline. Ten days before the end of modeling, rats of the pre-EA group received EA stimulation of LI11 and SP10 for 20 min, once a day for 10 consecutive days, and those of the medication group received gavage of loratadine tablets di