Establishment of fluorescence labeling and capillary electrophoresis in MSAP for Beijing You chicken

The DNA methylated states in muscle tissues from Beijing You chicken were analyzed using methylation sensitive amplified polymorphism (MSAP) combined with fluorescence labeling and capillary electrophoresis technology. We optimized the MSAP experimental condition including the genomic DNA concentrat...

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Bibliographic Details
Published inYíchuán Vol. 36; no. 5; p. 495
Main Authors Jinlong, Li, Shaoqing, Tang, Zhiyuan, Zou, Haichao, Wang, Qing, Xu
Format Journal Article
LanguageChinese
Published China 01.05.2014
Subjects
Animals
Chickens - genetics
DNA Methylation
Electrophoresis, Capillary - methods
Fluorescent Dyes - metabolism
Genomics
Nucleic Acid Amplification Techniques - methods
Staining and Labeling
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Summary:The DNA methylated states in muscle tissues from Beijing You chicken were analyzed using methylation sensitive amplified polymorphism (MSAP) combined with fluorescence labeling and capillary electrophoresis technology. We optimized the MSAP experimental condition including the genomic DNA concentration, dilution of pre-amplification prod-uct, the internal standard content and the concentrations of selective primers, Mg2+ and dNTPs. Repeated experiments showed that this method can automatically detect the global DNA methylation states in Beijing You chicken, and can be extended to other animals or plants with complex genomes and rich methylation polymorphism.
ISSN:0253-9772