Effect of endothelial progenitor cell on hematopoietic reconstitution in allogeneic hematopoietic stem cell transplantation mouse model

• Published in: Zhōnghuá xuèyèxué zázhì Vol. 34; no. 6; p. 516
• Main Authors: Hua, Jing, Fang, Ting, Liu, Mi-mi, Huang, Yu-jin, Fu, Jin-yu, Wu, Jin-yan, Xu, Kai-lin, Zeng, Ling-yu
• Format: Journal Article
• Language: Chinese
• Published: China 01.06.2013
• Subjects: Animals; Endothelial Cells - cytology; Hematopoietic Stem Cell Transplantation - methods; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Stem Cells - cytology; Transplantation, Homologous
• ISSN: 0253-2727
• DOI: 10.3760/cma.j.issn.0253-2727.2013.06.013

To examine the effects of endothelial progenitor cell (EPC) on hematopoietic reconstitution in allogeneic hematopoietic stem cell transplantation (allo-HSCT) mouse model. Allo-HSCT mouse model was established with condition of BU/CY, in which C57BL/6 (H-2b) and BALB/c (H-2d) mice were used as donors and recipients respectively. Recipients were randomly divided into 4 groups: untreated group, BU/CY condition group, bone marrow transplantation (BMT) group and transplantation of BM cells combined with EPCs (combined transplantation) group. The pathological changes of BM cells following transplantation were dynamically observed. Changes of BM sinusoidal endothelium and angiogenesis were observed by MECA-32 antibody immunohistochemical staining. The proportion of intramedullary stem and progenitor cells and serum cytokines were analyzed by flow cytometry. The numbers of peripheral blood cells were also counted. (1) Injuries of BM hematopoietic tissue, sinusoidal endothelium and vascular were less severe in combine