Morphology and cell proliferation evaluation of follicles from cryopreserved human ovarian tissue by vitrification

To evaluate the morphology and proliferation of follicles from cryopreserved human ovarian tissue by vitrification. Ovarian biopsy specimens were taken from 12 patients. The specimens were randomly distributed into fresh group (Group A) and vitrification group (Group B). Histological examination and...

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Published inZhejiang da xue xue bao. Journal of Zhejiang University. Medical sciences. Yi xue ban Vol. 42; no. 1; p. 75
Main Authors Pan, Yongmiao, Xu, Xiangrong, Qian, Yuli, Zhou, Caiyun, Xu, Jian
Format Journal Article
LanguageChinese
Published China 01.01.2013
Subjects
Adult
Cell Proliferation
Cryopreservation - methods
Female
Humans
In Vitro Techniques
Oocytes - cytology
Ovarian Follicle - cytology
Ovarian Follicle - ultrastructure
Ovary - anatomy & histology
Vitrification
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Summary:To evaluate the morphology and proliferation of follicles from cryopreserved human ovarian tissue by vitrification. Ovarian biopsy specimens were taken from 12 patients. The specimens were randomly distributed into fresh group (Group A) and vitrification group (Group B). Histological examination and ultrastructural observation were performed after cryopreservation. Both were embedded in paraffin block and proliferating cell nuclear antigen (PCNA) was detected by immunohistochemical staining. The proportions of primordial and primary follicles from Group A and Group B were 86.4%, 13.6% and 84.5%, 15.5%, respectively (P>0.05). There was no significant difference in proportions of morphologically normal primordial follicles between Group A and Group B (P>0.05); but the proportion of morphologically abnormal primary follicles was significantly higher in Group B than that in Group A (P<0.05). The ultrastructural studies showed that in histologically normal primordial follicles, there was no difference between Grou
ISSN:1008-9292