HPLC fingerprint spectrum of xinjiang uyghur medicine Nigella glandulifera seed

To establish the HPLC fingerprint spectrum of Nigella glandulifera seed identification. The chromatographic system consisted of a Shim-pack ODS C18 column (150 mm x 4.6 mm, 5 microm) and a mobile phase of acetonitrile-0.1% formic acid in condition of gradient elution,The detection wavelength was 280...

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Bibliographic Details
Published inZhong yao cai Vol. 34; no. 12; p. 1857
Main Authors Tan, Xiu-Fang, Li, Xiao-Jin, Wang, Qiang, Shi, Ming-Hui, Yang, Jie, Cao, Yuan
Format Journal Article
LanguageChinese
Published China 01.12.2011
Subjects
China
Chromatography, High Pressure Liquid - methods
Drugs, Chinese Herbal - chemistry
Drugs, Chinese Herbal - isolation & purification
Drugs, Chinese Herbal - standards
Nigella - chemistry
Plants, Medicinal - chemistry
Quality Control
Reproducibility of Results
Seeds - chemistry
Solvents - chemistry
China
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Summary:To establish the HPLC fingerprint spectrum of Nigella glandulifera seed identification. The chromatographic system consisted of a Shim-pack ODS C18 column (150 mm x 4.6 mm, 5 microm) and a mobile phase of acetonitrile-0.1% formic acid in condition of gradient elution,The detection wavelength was 280 nm. The column temperature was 30 degrees C. Detected the HPLC fingerprint spectrum of 10 batches samples Nigella glandulifera seed from Xinjiang; Calibrated 18 characteristic peaks. The result was in accordance with the technical requirement of fingerprint spectrum. The similarity calculation software showed that the similarity was higher than 0. 95. The HPLC fingerprint spectrum of Nigella glandulifera seed can be used as an identification method. It may provide the basis for quality control of Nigella glandulifera seed.
ISSN:1001-4454