Expression and clinical significance of HMGA2 in renal carcinoma

• Published in: Zhōnghuá zhŏngliú zázhì Vol. 39; no. 2; pp. 127 - 132
• Main Authors: Liu, Y, Fu, Q Z, Pu, L, Song, L L, Lyu, G Y, Liu, J, Wang, Zhenlong, Wang, Ziming
• Format: Journal Article
• Language: Chinese
• Published: China 23.02.2017
• Subjects: Blotting, Western; Carcinoma, Renal Cell - metabolism; Carcinoma, Renal Cell - secondary; Female; HMGA2 Protein - metabolism; Humans; Immunohistochemistry; Kidney Neoplasms - metabolism; Lymphatic Metastasis; Male; Neoplasm Staging; Prognosis; RNA, Messenger - metabolism; High mobility group A2; Immunohistochemistry; Clinicopathological features; Prognosis; Diagnosis; Renal neoplasms
• ISSN: 0253-3766
• DOI: 10.3760/cma.j.issn.0253-3766.2017.02.010

To detect the high mobility group A2 (HMGA2) expression in renal carcinoma, and to explore the relationship with clinicopathological features and its significance for prognosis. 50 renal carcinoma specimens, 50 corresponding adjacent normal kidney tissue samples, and 40 benign renal tumor specimens were used in this study. The expressions of HMGA2 mRNA and protein were detected by RT-PCR, Western blot and immunohistochemical assays, and its relationship with clinicopathological features and prognosis in the renal carcinoma patients was analyzed. The RT-PCR results showed that the relative expression levels of HMGA2 mRNA in the renal carcinoma, benign renal tumor tissues, and adjacent normal renal tissues were 0.84±0.23, 0.19± 0.06 and 0.08±0.04, respectively, and the expression in renal carcinoma tissue was significantly higher than those of the other 2 groups ( <0.01). The Western blot results showed that the relative expression levels of HMGA2 protein in the renal carcinoma, benign renal tumor tissues, and